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***Immobilized pH gradient*** (IPG) is another variation of isofocusing that uses a gel with a pre-cast pH gradient. In IPG gels, the pH gradient is chemically bound to the gel matrix, which prevents it from drifting during the experiment. This results in more stable and reproducible isofocusing, especially for long separation times. IPG gels are commercially available in a wide range of pH gradients, allowing researchers to select the optimal gradient for their specific protein mixture. The use of IPG gels simplifies the experimental setup and improves the reliability of the isofocusing process. These different techniques each offer unique advantages depending on the specific research or diagnostic goals.
